ssr_primer
Primer Details for TRINITY_DN86831_c5_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGAGCGTGAATTGATGAG | ACGCGAAGTGAGAGCTTGAA | Start: 243 |
| Tm | 60.044 | 59.968 | End: 493 |
| Size | 20 | 20 | 251 |
| Primer 2 details |
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| Sequence | CGCGAGCGTGAATTGATGAG | AACGCGAAGTGAGAGCTTGA | Start: 243 |
| Tm | 60.044 | 59.968 | End: 494 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | TCTTCTGGCTCCACGGAAAC | CTCATCAATTCACGCTCGCG | Start: 90 |
| Tm | 59.965 | 60.044 | End: 262 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
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| Sequence | TCACATTTTCTCGTCGGCGA | CTCATCAATTCACGCTCGCG | Start: 150 |
| Tm | 60.039 | 60.044 | End: 262 |
| Size | 20 | 20 | 113 |
| Primer 5 details |
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| Sequence | CACGGAAACAGCAGCAACAA | CTCATCAATTCACGCTCGCG | Start: 101 |
| Tm | 59.9 | 60.044 | End: 262 |
| Size | 20 | 20 | 162 |
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