ssr_primer
Primer Details for TRINITY_DN86830_c4_g1_i2 |
|---|
| (T)14: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAATCGACACCATGGGCA | TGTAGCGATGGGGTAGCTCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 151 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | TCAAATCGACACCATGGGCA | TCTGTAGCGATGGGGTAGCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 153 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TGTAGCGATGGGGTAGCTCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TCTGTAGCGATGGGGTAGCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 153 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | AATCGACACCATGGGCAGTT | TGTAGCGATGGGGTAGCTCT | Start: 12 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 140 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAATCGACACCATGGGCA | TGTAGCGATGGGGTAGCTCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 151 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | TCAAATCGACACCATGGGCA | TCTGTAGCGATGGGGTAGCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 153 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TGTAGCGATGGGGTAGCTCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TCTGTAGCGATGGGGTAGCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 153 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | AATCGACACCATGGGCAGTT | TGTAGCGATGGGGTAGCTCT | Start: 12 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 140 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAAATCGACACCATGGGCA | TGTAGCGATGGGGTAGCTCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 151 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
|---|
| Sequence | TCAAATCGACACCATGGGCA | TCTGTAGCGATGGGGTAGCT | Start: 9 |
| Tm | 59.963 | 60.106 | End: 153 |
| Size | 20 | 20 | 145 |
| Primer 3 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TGTAGCGATGGGGTAGCTCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 139 |
| Primer 4 details |
|---|
| Sequence | ATCGACACCATGGGCAGTTT | TCTGTAGCGATGGGGTAGCT | Start: 13 |
| Tm | 59.962 | 60.106 | End: 153 |
| Size | 20 | 20 | 141 |
| Primer 5 details |
|---|
| Sequence | AATCGACACCATGGGCAGTT | TGTAGCGATGGGGTAGCTCT | Start: 12 |
| Tm | 59.962 | 60.106 | End: 151 |
| Size | 20 | 20 | 140 |
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