Primer Details for TRINITY_DN86827_c1_g5_i1 | |||
|---|---|---|---|
| (T)10: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCATTCTACCACAAGACTTCACCT | AAATTCACCCACCTACGCCT | Start: 3 |
| Tm | 59.407 | 59.303 | End: 146 |
| Size | 24 | 20 | 144 |
| Primer 2 details | |||
| Sequence | TCATTCTACCACAAGACTTCACC | AAATTCACCCACCTACGCCT | Start: 3 |
| Tm | 58.105 | 59.303 | End: 146 |
| Size | 23 | 20 | 144 |
| Primer 3 details | |||
| Sequence | CATTCTACCACAAGACTTCACCT | AAATTCACCCACCTACGCCT | Start: 4 |
| Tm | 58.1 | 59.303 | End: 146 |
| Size | 23 | 20 | 143 |
| Primer 4 details | |||
| Sequence | TCATTCTACCACAAGACTTCACCT | AAAATTCACCCACCTACGCCT | Start: 3 |
| Tm | 59.407 | 59.925 | End: 147 |
| Size | 24 | 21 | 145 |
| Primer 5 details | |||
| Sequence | TTCATTCTACCACAAGACTTCACCT | AAATTCACCCACCTACGCCT | Start: 2 |
| Tm | 59.928 | 59.303 | End: 146 |
| Size | 25 | 20 | 145 |
