ssr_primer
Primer Details for TRINITY_DN86818_c2_g1_i1 |
|---|
| (TC)7: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATGAAGCCTCCCCTAA | TGCTACCCTCACAACGTTCC | Start: 529 |
| Tm | 60.032 | 59.966 | End: 781 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | CCTGCTACCCTCACAACGTT | Start: 529 |
| Tm | 60.032 | 59.965 | End: 783 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CCACCTCCACAGCCATTCTT | CACCAGTTTTCACCACTGCG | Start: 998 |
| Tm | 59.961 | 59.971 | End: 1127 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GGTCTTCCACTCTGACAGCC | TTAGGGGAGGCTTCATCGGA | Start: 348 |
| Tm | 60.037 | 60.032 | End: 548 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | GCTGAGGCGTAGGTGTTCTT | Start: 529 |
| Tm | 60.032 | 60.037 | End: 653 |
| Size | 20 | 20 | 125 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATGAAGCCTCCCCTAA | TGCTACCCTCACAACGTTCC | Start: 529 |
| Tm | 60.032 | 59.966 | End: 781 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | CCTGCTACCCTCACAACGTT | Start: 529 |
| Tm | 60.032 | 59.965 | End: 783 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CCACCTCCACAGCCATTCTT | CACCAGTTTTCACCACTGCG | Start: 998 |
| Tm | 59.961 | 59.971 | End: 1127 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GGTCTTCCACTCTGACAGCC | TTAGGGGAGGCTTCATCGGA | Start: 348 |
| Tm | 60.037 | 60.032 | End: 548 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | GCTGAGGCGTAGGTGTTCTT | Start: 529 |
| Tm | 60.032 | 60.037 | End: 653 |
| Size | 20 | 20 | 125 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATGAAGCCTCCCCTAA | TGCTACCCTCACAACGTTCC | Start: 529 |
| Tm | 60.032 | 59.966 | End: 781 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | CCTGCTACCCTCACAACGTT | Start: 529 |
| Tm | 60.032 | 59.965 | End: 783 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CCACCTCCACAGCCATTCTT | CACCAGTTTTCACCACTGCG | Start: 998 |
| Tm | 59.961 | 59.971 | End: 1127 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GGTCTTCCACTCTGACAGCC | TTAGGGGAGGCTTCATCGGA | Start: 348 |
| Tm | 60.037 | 60.032 | End: 548 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | GCTGAGGCGTAGGTGTTCTT | Start: 529 |
| Tm | 60.032 | 60.037 | End: 653 |
| Size | 20 | 20 | 125 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCGATGAAGCCTCCCCTAA | TGCTACCCTCACAACGTTCC | Start: 529 |
| Tm | 60.032 | 59.966 | End: 781 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | CCTGCTACCCTCACAACGTT | Start: 529 |
| Tm | 60.032 | 59.965 | End: 783 |
| Size | 20 | 20 | 255 |
| Primer 3 details |
|---|
| Sequence | CCACCTCCACAGCCATTCTT | CACCAGTTTTCACCACTGCG | Start: 998 |
| Tm | 59.961 | 59.971 | End: 1127 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | GGTCTTCCACTCTGACAGCC | TTAGGGGAGGCTTCATCGGA | Start: 348 |
| Tm | 60.037 | 60.032 | End: 548 |
| Size | 20 | 20 | 201 |
| Primer 5 details |
|---|
| Sequence | TCCGATGAAGCCTCCCCTAA | GCTGAGGCGTAGGTGTTCTT | Start: 529 |
| Tm | 60.032 | 60.037 | End: 653 |
| Size | 20 | 20 | 125 |
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