ssr_primer
Primer Details for TRINITY_DN86811_c2_g1_i13 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTTGGTTTGACGAGCA | CAGAGCCACTGTGACCTCTG | Start: 610 |
| Tm | 60.107 | 60.038 | End: 855 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | GGGCAACAAAGCTTCAGAGC | TGCTCGTCAAACCAAGGTGT | Start: 449 |
| Tm | 60.039 | 60.107 | End: 629 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | ACCTTGGTTTGACGAGCACT | CAGAGCCACTGTGACCTCTG | Start: 612 |
| Tm | 59.82 | 60.038 | End: 855 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GGGCAACAAAGCTTCAGAGC | AGTGCTCGTCAAACCAAGGT | Start: 449 |
| Tm | 60.039 | 59.82 | End: 631 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | CACCTTGGTTTGACGAGCAC | CAGAGCCACTGTGACCTCTG | Start: 611 |
| Tm | 59.692 | 60.038 | End: 855 |
| Size | 20 | 20 | 245 |
(TAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCTTGGTTTGACGAGCA | CAGAGCCACTGTGACCTCTG | Start: 610 |
| Tm | 60.107 | 60.038 | End: 855 |
| Size | 20 | 20 | 246 |
| Primer 2 details |
|---|
| Sequence | GGGCAACAAAGCTTCAGAGC | TGCTCGTCAAACCAAGGTGT | Start: 449 |
| Tm | 60.039 | 60.107 | End: 629 |
| Size | 20 | 20 | 181 |
| Primer 3 details |
|---|
| Sequence | ACCTTGGTTTGACGAGCACT | CAGAGCCACTGTGACCTCTG | Start: 612 |
| Tm | 59.82 | 60.038 | End: 855 |
| Size | 20 | 20 | 244 |
| Primer 4 details |
|---|
| Sequence | GGGCAACAAAGCTTCAGAGC | AGTGCTCGTCAAACCAAGGT | Start: 449 |
| Tm | 60.039 | 59.82 | End: 631 |
| Size | 20 | 20 | 183 |
| Primer 5 details |
|---|
| Sequence | CACCTTGGTTTGACGAGCAC | CAGAGCCACTGTGACCTCTG | Start: 611 |
| Tm | 59.692 | 60.038 | End: 855 |
| Size | 20 | 20 | 245 |
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