ssr_primer
Primer Details for TRINITY_DN86805_c1_g2_i2 |
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| (T)29: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCAAACTTGATCGGCCGTA | ACACGCTTACCGCAGAGAAA | Start: 420 |
| Tm | 60.037 | 59.967 | End: 653 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
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| Sequence | TGGAAGACCTGCGTTTCTCC | GCACATCCAGGGTCCTTCAA | Start: 1715 |
| Tm | 59.965 | 59.962 | End: 1981 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
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| Sequence | GCAAACTTGATCGGCCGTAC | ACACGCTTACCGCAGAGAAA | Start: 421 |
| Tm | 59.904 | 59.967 | End: 653 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
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| Sequence | CCACGCCCAATCGAATGTTC | ACACGCTTACCGCAGAGAAA | Start: 400 |
| Tm | 59.901 | 59.967 | End: 653 |
| Size | 20 | 20 | 254 |
| Primer 5 details |
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| Sequence | AGCAAACTTGATCGGCCGTA | GTACACGCTTACCGCAGAGA | Start: 420 |
| Tm | 60.037 | 59.83 | End: 655 |
| Size | 20 | 20 | 236 |
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