ssr_primer
Primer Details for TRINITY_DN86792_c0_g1_i3 |
|---|
| (A)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAGGCACCGAATGAGT | TGCACAAAGAACGCTCCTCT | Start: 1928 |
| Tm | 59.891 | 59.893 | End: 2050 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
|---|
| Sequence | CATTACACTGCGGGGGACAT | GAAAGCGGCCACAAACTAGC | Start: 124 |
| Tm | 60.107 | 60.11 | End: 386 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AAGGACACTTTCTCACCCGC | TGCACAAAGAACGCTCCTCT | Start: 1878 |
| Tm | 60.25 | 59.893 | End: 2050 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | CAAGATCAGCAGCGGGGTAA | GCGGGTGAGAAAGTGTCCTT | Start: 1630 |
| Tm | 60.108 | 60.25 | End: 1897 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | CCGAATTTGCCGGTTGGTTT | AAATCCTAGCAGGTGCCACG | Start: 1560 |
| Tm | 59.967 | 60.393 | End: 1671 |
| Size | 20 | 20 | 112 |
(A)19(ACAACAACAACAACAACAATAACAACAAATTGCGGATGTGATAATTATGCATTATAGTACAAAAAGTTTGAGAGTAAAAAAATAGACAAAAACCAACTCCTTACAGTGCAAAAAGATACTCAATAATTTACTTTCTCAATCTTATTCATACCGATCACCATGCAGCCACCCTCGCTTACTCGTAAAAACGAATGACCCAAGTAGCAGAGACCGCCATAAGTTGCAAAAATATTGTCAAATTATTGCAATTTTGTGGCAAGATATGTGGATTGCTTATTTTCCTCTTTGGAAGGCCAAGATTATGAACATTCATTGCAATAATATTACAATAAATTGGAATAAGGGGGCAATAGTTTGCAATTGCTGGTTGTGATGCAGATTGCCTACCTCTTTTAGTCAAGAAACCCTCGAGGTTGGCTATGAAATCGCCAAAAATCGTGGAAAATTGTCCCTATTTTTTCCAAAATTGCAGTTTGATTGCAACATTATGACAATACGTTGAAATATTATTACTACTTTAACTTTGTAAAATACTACTTAGAGAGTAGAAATACTCACAGGTGTTAGTCCGTAGTCGTTTCCGAGAGTTACATAGTTATCTTGAGCTTTGACTAAGGTGATTTCTCCGCCGAATTTGCCGGTTGGTTTGTATGCATCAGCAGCGACTAATTTCTTGTAGAAGGACTCCGCAGCAGTGGCAAGATCAGCAGCGGGGTAAGGCGTGGCACCTGCTAGGATTTCTGTCACTTTCTTCAGTCGGGATTCGTAACTAGGTAAGGATGACAGTTCCTTCTGGGTCTTTTGGTAGTCAACATCCTTGAACAATTGAATGAAGTACGTTAAAGCATCAGCATCGCCAGCTTGTTGTCCACCTTTCTGTTCCTTTTTCTGCTTGTAGCTTCCGGTATGACTGGCAACATAGTCAGGTGAACCGTCTAATAGAACTAAGGACACTTTCTCACCCGCAGCTTCTAGCTGGAGACCCATCTCAAATGCAACACAGGCACCGAATGAGTATCCAGCTAACGAGTAGGGACCAGATTTTTGTTTCCTTTTCACTTCCTGAATATAAGAAGCGGCTAAGTCTTGAATAGAGCTAAGAGGAGCGTTCTTTGTGCC)(AAC)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACAGGCACCGAATGAGT | TGCACAAAGAACGCTCCTCT | Start: 1928 |
| Tm | 59.891 | 59.893 | End: 2050 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
|---|
| Sequence | CATTACACTGCGGGGGACAT | GAAAGCGGCCACAAACTAGC | Start: 124 |
| Tm | 60.107 | 60.11 | End: 386 |
| Size | 20 | 20 | 263 |
| Primer 3 details |
|---|
| Sequence | AAGGACACTTTCTCACCCGC | TGCACAAAGAACGCTCCTCT | Start: 1878 |
| Tm | 60.25 | 59.893 | End: 2050 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | CAAGATCAGCAGCGGGGTAA | GCGGGTGAGAAAGTGTCCTT | Start: 1630 |
| Tm | 60.108 | 60.25 | End: 1897 |
| Size | 20 | 20 | 268 |
| Primer 5 details |
|---|
| Sequence | CCGAATTTGCCGGTTGGTTT | AAATCCTAGCAGGTGCCACG | Start: 1560 |
| Tm | 59.967 | 60.393 | End: 1671 |
| Size | 20 | 20 | 112 |
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