ssr_primer
Primer Details for TRINITY_DN86771_c3_g1_i1 |
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| (G)18c(G)28cgcgc(G)18: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGAGGGGAGAAGAAACA | CTCCAGCGAACCACTAGCAA | Start: 79 |
| Tm | 60.845 | 60.037 | End: 222 |
| Size | 20 | 20 | 144 |
| Primer 2 details |
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| Sequence | GGGGAGGGGAGAAGAAACAA | CTCCAGCGAACCACTAGCAA | Start: 80 |
| Tm | 58.927 | 60.037 | End: 222 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | GGGGGAGGGGAGAAGAAACA | TCCAGCGAACCACTAGCAAC | Start: 79 |
| Tm | 60.845 | 60.32 | End: 221 |
| Size | 20 | 20 | 143 |
| Primer 4 details |
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| Sequence | GGGGGAGGGGAGAAGAAACA | CCACTAGCAACACGCGAGAT | Start: 79 |
| Tm | 60.845 | 60.459 | End: 212 |
| Size | 20 | 20 | 134 |
| Primer 5 details |
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| Sequence | GGGGGAGGGGAGAAGAAACA | CAGCGAACCACTAGCAACAC | Start: 79 |
| Tm | 60.845 | 59.487 | End: 219 |
| Size | 20 | 20 | 141 |
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