ssr_primer
Primer Details for TRINITY_DN86731_c0_g1_i1 |
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| (T)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAGTTGCGGCGTCATCAAAA | AAAAAGAGGTAGGCTGGGGC | Start: 7 |
| Tm | 59.763 | 59.96 | End: 111 |
| Size | 20 | 20 | 105 |
| Primer 2 details |
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| Sequence | GAGTTGCGGCGTCATCAAAA | CTGTTTCTCCCACCACGGAA | Start: 7 |
| Tm | 59.763 | 59.892 | End: 130 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
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| Sequence | GAGTTGCGGCGTCATCAAAA | ATTCACCGGCTGTTTCTCCC | Start: 7 |
| Tm | 59.763 | 60.323 | End: 139 |
| Size | 20 | 20 | 133 |
| Primer 4 details |
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| Sequence | TTCCGTGGTGGGAGAAACAG | AAAAGAAACGCGAAGGAGGC | Start: 111 |
| Tm | 59.892 | 59.408 | End: 390 |
| Size | 20 | 20 | 280 |
| Primer 5 details |
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| Sequence | TTCCGTGGTGGGAGAAACAG | CGCGAAGGAGGCCAGTTTAA | Start: 111 |
| Tm | 59.892 | 60.672 | End: 382 |
| Size | 20 | 20 | 272 |
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