ssr_primer
Primer Details for TRINITY_DN86728_c3_g1_i4 |
|---|
| (T)43: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCAACGCTAAGAACGACT | TTGGGTTTTTGACGACCGGA | Start: 403 |
| Tm | 59.967 | 60.107 | End: 504 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TGGCAACGCTAAGAACGACT | TGTGATACAAGCACGCACCA | Start: 403 |
| Tm | 59.967 | 60.25 | End: 560 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | TGGTGCGTGCTTGTATCACA | CCTTGAAGTCGGGGAAGTCC | Start: 541 |
| Tm | 60.25 | 60.036 | End: 780 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
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| Sequence | TCCGGTCGTCAAAAACCCAA | GCAAGACAACTCCTTGACGC | Start: 485 |
| Tm | 60.107 | 59.764 | End: 753 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | ACACAAGGGTCCAAAGGGTG | TGTGATACAAGCACGCACCA | Start: 460 |
| Tm | 60.106 | 60.25 | End: 560 |
| Size | 20 | 20 | 101 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGCAACGCTAAGAACGACT | TTGGGTTTTTGACGACCGGA | Start: 403 |
| Tm | 59.967 | 60.107 | End: 504 |
| Size | 20 | 20 | 102 |
| Primer 2 details |
|---|
| Sequence | TGGCAACGCTAAGAACGACT | TGTGATACAAGCACGCACCA | Start: 403 |
| Tm | 59.967 | 60.25 | End: 560 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
|---|
| Sequence | TGGTGCGTGCTTGTATCACA | CCTTGAAGTCGGGGAAGTCC | Start: 541 |
| Tm | 60.25 | 60.036 | End: 780 |
| Size | 20 | 20 | 240 |
| Primer 4 details |
|---|
| Sequence | TCCGGTCGTCAAAAACCCAA | GCAAGACAACTCCTTGACGC | Start: 485 |
| Tm | 60.107 | 59.764 | End: 753 |
| Size | 20 | 20 | 269 |
| Primer 5 details |
|---|
| Sequence | ACACAAGGGTCCAAAGGGTG | TGTGATACAAGCACGCACCA | Start: 460 |
| Tm | 60.106 | 60.25 | End: 560 |
| Size | 20 | 20 | 101 |
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