ssr_primer
Primer Details for TRINITY_DN86721_c0_g1_i6 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGGCAAAACGGGTCATAT | TACCTCCCAATCCAAACGGC | Start: 3477 |
| Tm | 60.035 | 60.035 | End: 3583 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
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| Sequence | TTCAGGTTGATCGACGGTGG | ATATGACCCGTTTTGCCCGT | Start: 3288 |
| Tm | 60.038 | 60.035 | End: 3496 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
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| Sequence | CAAAACCGACCGGCTCTTTG | GCTTCTGTTGTCGCTTTGGG | Start: 695 |
| Tm | 60.04 | 60.04 | End: 866 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
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| Sequence | GAGTTCCCGCAAGTAGTCCC | CCACCGTCGATCAACCTGAA | Start: 3190 |
| Tm | 60.108 | 60.038 | End: 3307 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
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| Sequence | CAAAACCGACCGGCTCTTTG | TGGAAGCTTCTGTTGTCGCT | Start: 695 |
| Tm | 60.04 | 59.893 | End: 871 |
| Size | 20 | 20 | 177 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGGGCAAAACGGGTCATAT | TACCTCCCAATCCAAACGGC | Start: 3477 |
| Tm | 60.035 | 60.035 | End: 3583 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
|---|
| Sequence | TTCAGGTTGATCGACGGTGG | ATATGACCCGTTTTGCCCGT | Start: 3288 |
| Tm | 60.038 | 60.035 | End: 3496 |
| Size | 20 | 20 | 209 |
| Primer 3 details |
|---|
| Sequence | CAAAACCGACCGGCTCTTTG | GCTTCTGTTGTCGCTTTGGG | Start: 695 |
| Tm | 60.04 | 60.04 | End: 866 |
| Size | 20 | 20 | 172 |
| Primer 4 details |
|---|
| Sequence | GAGTTCCCGCAAGTAGTCCC | CCACCGTCGATCAACCTGAA | Start: 3190 |
| Tm | 60.108 | 60.038 | End: 3307 |
| Size | 20 | 20 | 118 |
| Primer 5 details |
|---|
| Sequence | CAAAACCGACCGGCTCTTTG | TGGAAGCTTCTGTTGTCGCT | Start: 695 |
| Tm | 60.04 | 59.893 | End: 871 |
| Size | 20 | 20 | 177 |
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