ssr_primer
Primer Details for TRINITY_DN86720_c2_g1_i2 |
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| (T)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAGGACTCTGATGCGGACT | GTGCTCTGGCTAAGCTGGAA | Start: 3182 |
| Tm | 60.034 | 60.036 | End: 3284 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | AGAGGACTCTGATGCGGACT | ACAGTGCTCTGGCTAAGCTG | Start: 3182 |
| Tm | 60.034 | 60.037 | End: 3287 |
| Size | 20 | 20 | 106 |
| Primer 3 details |
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| Sequence | AGGAAGTTAAGGGCAGGCAC | ATCGCCAAACCGAAGACTGT | Start: 4703 |
| Tm | 59.962 | 59.965 | End: 4919 |
| Size | 20 | 20 | 217 |
| Primer 4 details |
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| Sequence | TTCCAGCTTAGCCAGAGCAC | GGCAAAATCCCTCTCCCCAA | Start: 3265 |
| Tm | 60.036 | 59.959 | End: 3517 |
| Size | 20 | 20 | 253 |
| Primer 5 details |
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| Sequence | CAGCTTAGCCAGAGCACTGT | GGCAAAATCCCTCTCCCCAA | Start: 3268 |
| Tm | 60.037 | 59.959 | End: 3517 |
| Size | 20 | 20 | 250 |
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