ssr_primer
Primer Details for TRINITY_DN86720_c1_g1_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGCGCCCACTTCTTTTTG | TTGACGAGCTTTACGCCAGT | Start: 132 |
| Tm | 59.898 | 59.967 | End: 359 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
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| Sequence | GTGTGCGCCCACTTCTTTTT | TTGACGAGCTTTACGCCAGT | Start: 131 |
| Tm | 59.898 | 59.967 | End: 359 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
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| Sequence | GAGCTTGACAACAGGGGGTT | CAAAAAGAAGTGGGCGCACA | Start: 4 |
| Tm | 60.179 | 59.898 | End: 151 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
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| Sequence | GAGCTTGACAACAGGGGGTT | AAAAAGAAGTGGGCGCACAC | Start: 4 |
| Tm | 60.179 | 59.898 | End: 150 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | ATATCGGTTTCGGCCCTTCG | TTGACGAGCTTTACGCCAGT | Start: 152 |
| Tm | 60.25 | 59.967 | End: 359 |
| Size | 20 | 20 | 208 |
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