ssr_primer
Primer Details for TRINITY_DN86689_c0_g3_i1 |
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| (A)20cgcattagtttgcaagctcctgaa(T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATGAGGACACGGCAAA | TGGCCAAGGGAATCGGAAAA | Start: 1492 |
| Tm | 59.966 | 59.887 | End: 1609 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTCGATGAGGACACGGCAAA | TTGGCCAAGGGAATCGGAAA | Start: 1492 |
| Tm | 59.966 | 59.887 | End: 1610 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
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| Sequence | AAGAATACACCATCGGGGGC | TTTGCCGTGTCCTCATCGAA | Start: 1344 |
| Tm | 59.817 | 59.966 | End: 1511 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
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| Sequence | GGGGCGAATTGAATCCTCCT | TTTGCCGTGTCCTCATCGAA | Start: 1359 |
| Tm | 59.816 | 59.966 | End: 1511 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
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| Sequence | TGCGGCCGAAAAATACACAC | GAAGAGCTGTTCCTGTGCCT | Start: 183 |
| Tm | 59.761 | 59.964 | End: 318 |
| Size | 20 | 20 | 136 |
(A)10tgtactcacttgaagtatctctttggtgtcatagcagaa(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCGATGAGGACACGGCAAA | TGGCCAAGGGAATCGGAAAA | Start: 1492 |
| Tm | 59.966 | 59.887 | End: 1609 |
| Size | 20 | 20 | 118 |
| Primer 2 details |
|---|
| Sequence | TTCGATGAGGACACGGCAAA | TTGGCCAAGGGAATCGGAAA | Start: 1492 |
| Tm | 59.966 | 59.887 | End: 1610 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | AAGAATACACCATCGGGGGC | TTTGCCGTGTCCTCATCGAA | Start: 1344 |
| Tm | 59.817 | 59.966 | End: 1511 |
| Size | 20 | 20 | 168 |
| Primer 4 details |
|---|
| Sequence | GGGGCGAATTGAATCCTCCT | TTTGCCGTGTCCTCATCGAA | Start: 1359 |
| Tm | 59.816 | 59.966 | End: 1511 |
| Size | 20 | 20 | 153 |
| Primer 5 details |
|---|
| Sequence | TGCGGCCGAAAAATACACAC | GAAGAGCTGTTCCTGTGCCT | Start: 183 |
| Tm | 59.761 | 59.964 | End: 318 |
| Size | 20 | 20 | 136 |
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