ssr_primer
Primer Details for TRINITY_DN86659_c5_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCACGGAATATGGGAGCTA | GGACCAGGCTGAATTCAGGT | Start: 208 |
| Tm | 59.683 | 59.67 | End: 350 |
| Size | 20 | 20 | 143 |
| Primer 2 details |
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| Sequence | GGAGCCGGGAGAAAAAGGAG | GCCTCTCGCTAAGTAGCTCC | Start: 68 |
| Tm | 60.393 | 59.687 | End: 240 |
| Size | 20 | 20 | 173 |
| Primer 3 details |
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| Sequence | GGAGCCGGGAGAAAAAGGAG | TAGCTCCCATATTCCGTGCG | Start: 68 |
| Tm | 60.393 | 59.683 | End: 227 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | GGAGCCGGGAGAAAAAGGAG | CGCTGCCTCTCGCTAAGTAG | Start: 68 |
| Tm | 60.393 | 60.317 | End: 244 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
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| Sequence | GTAAAAGCGAGGGGAGGGTG | GGACCAGGCTGAATTCAGGT | Start: 178 |
| Tm | 60.393 | 59.67 | End: 350 |
| Size | 20 | 20 | 173 |
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