ssr_primer
Primer Details for TRINITY_DN86599_c1_g1_i1 |
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| (G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGCGGAGGGTTATCAATC | TGTTGTTGCAATCTGGTGACA | Start: 86 |
| Tm | 60.251 | 58.627 | End: 187 |
| Size | 20 | 21 | 102 |
| Primer 2 details |
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| Sequence | TCCGTCTTTTAAGTGGCCCG | TGTTGTTGCAATCTGGTGACA | Start: 23 |
| Tm | 60.321 | 58.627 | End: 187 |
| Size | 20 | 21 | 165 |
| Primer 3 details |
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| Sequence | ACGGCGGGAAGAGTGTTTTT | TGTTGTTGCAATCTGGTGACA | Start: 50 |
| Tm | 60.465 | 58.627 | End: 187 |
| Size | 20 | 21 | 138 |
| Primer 4 details |
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| Sequence | GGGGCGGAGGGTTATCAATC | TTGTTGTTGCAATCTGGTGACA | Start: 86 |
| Tm | 60.251 | 59.243 | End: 188 |
| Size | 20 | 22 | 103 |
| Primer 5 details |
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| Sequence | TCCGTCTTTTAAGTGGCCCG | TTGTTGTTGCAATCTGGTGACA | Start: 23 |
| Tm | 60.321 | 59.243 | End: 188 |
| Size | 20 | 22 | 166 |
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