ssr_primer
Primer Details for TRINITY_DN86576_c2_g2_i1 |
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| (A)20: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCGAGGGAACTATTCACCC | TCTGACTTGACCGCGAGAAC | Start: 441 |
| Tm | 60.179 | 60.039 | End: 610 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
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| Sequence | GCCGAGGGAACTATTCACCC | GTCCATCGAGTTCGCCTTCA | Start: 441 |
| Tm | 60.179 | 60.109 | End: 665 |
| Size | 20 | 20 | 225 |
| Primer 3 details |
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| Sequence | GCCGAGGGAACTATTCACCC | TCCATCGAGTTCGCCTTCAG | Start: 441 |
| Tm | 60.179 | 59.827 | End: 664 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
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| Sequence | GTTCCGGTCCATCGAGAAGG | GGGTGAATAGTTCCCTCGGC | Start: 272 |
| Tm | 60.179 | 60.179 | End: 460 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
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| Sequence | GTTCTCGCGGTCAAGTCAGA | TTTCAACTCCTGAGCCGCTC | Start: 591 |
| Tm | 60.039 | 60.321 | End: 692 |
| Size | 20 | 20 | 102 |
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