ssr_primer
Primer Details for TRINITY_DN86558_c3_g2_i5 |
|---|
| (T)15: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCAAAACACGGTTCAGTC | CCTCGCAAGACCCAGAACAT | Start: 5806 |
| Tm | 59.97 | 60.036 | End: 6003 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CAGCCCAAAACACGGTTCAG | CCTCGCAAGACCCAGAACAT | Start: 5804 |
| Tm | 59.969 | 60.036 | End: 6003 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | AGGCGATTCGTTTCCCATGT | TGCTCCTGCGAACCGTAAAT | Start: 679 |
| Tm | 60.035 | 60.037 | End: 828 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACTCTACTAGCGCGGCTC | CTGGTGGGGGTGGCATTAAT | Start: 4304 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | GAAACTCTACTAGCGCGGCT | CTGGTGGGGGTGGCATTAAT | Start: 4303 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 178 |
(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCAAAACACGGTTCAGTC | CCTCGCAAGACCCAGAACAT | Start: 5806 |
| Tm | 59.97 | 60.036 | End: 6003 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CAGCCCAAAACACGGTTCAG | CCTCGCAAGACCCAGAACAT | Start: 5804 |
| Tm | 59.969 | 60.036 | End: 6003 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | AGGCGATTCGTTTCCCATGT | TGCTCCTGCGAACCGTAAAT | Start: 679 |
| Tm | 60.035 | 60.037 | End: 828 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACTCTACTAGCGCGGCTC | CTGGTGGGGGTGGCATTAAT | Start: 4304 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | GAAACTCTACTAGCGCGGCT | CTGGTGGGGGTGGCATTAAT | Start: 4303 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 178 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCCCAAAACACGGTTCAGTC | CCTCGCAAGACCCAGAACAT | Start: 5806 |
| Tm | 59.97 | 60.036 | End: 6003 |
| Size | 20 | 20 | 198 |
| Primer 2 details |
|---|
| Sequence | CAGCCCAAAACACGGTTCAG | CCTCGCAAGACCCAGAACAT | Start: 5804 |
| Tm | 59.969 | 60.036 | End: 6003 |
| Size | 20 | 20 | 200 |
| Primer 3 details |
|---|
| Sequence | AGGCGATTCGTTTCCCATGT | TGCTCCTGCGAACCGTAAAT | Start: 679 |
| Tm | 60.035 | 60.037 | End: 828 |
| Size | 20 | 20 | 150 |
| Primer 4 details |
|---|
| Sequence | AAACTCTACTAGCGCGGCTC | CTGGTGGGGGTGGCATTAAT | Start: 4304 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 177 |
| Primer 5 details |
|---|
| Sequence | GAAACTCTACTAGCGCGGCT | CTGGTGGGGGTGGCATTAAT | Start: 4303 |
| Tm | 59.899 | 60.032 | End: 4480 |
| Size | 20 | 20 | 178 |
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