ssr_primer
Primer Details for TRINITY_DN86557_c0_g1_i5 |
|---|
| (AAAG)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGGCTCGTGAATTTCGGC | CTCGAGTACGCCCAGATGAC | Start: 511 |
| Tm | 59.972 | 59.97 | End: 630 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | CGGCGGGAGTTAGACAACTT | GCCGAAATTCACGAGCCATC | Start: 260 |
| Tm | 60.038 | 59.972 | End: 530 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GTTTCGTCTGGTCGAGCTGA | GCCGAAATTCACGAGCCATC | Start: 331 |
| Tm | 60.039 | 59.972 | End: 530 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | GTCATCTGGGCGTACTCGAG | GCCGTGTCCGAAGAGAAGAA | Start: 611 |
| Tm | 59.97 | 60.039 | End: 807 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGCCAGCAAACTACCCCTC | ACGGAGATGGCGTTTGGATT | Start: 954 |
| Tm | 60.034 | 60.035 | End: 1110 |
| Size | 20 | 20 | 157 |
(AAAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGGCTCGTGAATTTCGGC | CTCGAGTACGCCCAGATGAC | Start: 511 |
| Tm | 59.972 | 59.97 | End: 630 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | CGGCGGGAGTTAGACAACTT | GCCGAAATTCACGAGCCATC | Start: 260 |
| Tm | 60.038 | 59.972 | End: 530 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GTTTCGTCTGGTCGAGCTGA | GCCGAAATTCACGAGCCATC | Start: 331 |
| Tm | 60.039 | 59.972 | End: 530 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | GTCATCTGGGCGTACTCGAG | GCCGTGTCCGAAGAGAAGAA | Start: 611 |
| Tm | 59.97 | 60.039 | End: 807 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGCCAGCAAACTACCCCTC | ACGGAGATGGCGTTTGGATT | Start: 954 |
| Tm | 60.034 | 60.035 | End: 1110 |
| Size | 20 | 20 | 157 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GATGGCTCGTGAATTTCGGC | CTCGAGTACGCCCAGATGAC | Start: 511 |
| Tm | 59.972 | 59.97 | End: 630 |
| Size | 20 | 20 | 120 |
| Primer 2 details |
|---|
| Sequence | CGGCGGGAGTTAGACAACTT | GCCGAAATTCACGAGCCATC | Start: 260 |
| Tm | 60.038 | 59.972 | End: 530 |
| Size | 20 | 20 | 271 |
| Primer 3 details |
|---|
| Sequence | GTTTCGTCTGGTCGAGCTGA | GCCGAAATTCACGAGCCATC | Start: 331 |
| Tm | 60.039 | 59.972 | End: 530 |
| Size | 20 | 20 | 200 |
| Primer 4 details |
|---|
| Sequence | GTCATCTGGGCGTACTCGAG | GCCGTGTCCGAAGAGAAGAA | Start: 611 |
| Tm | 59.97 | 60.039 | End: 807 |
| Size | 20 | 20 | 197 |
| Primer 5 details |
|---|
| Sequence | ATGCCAGCAAACTACCCCTC | ACGGAGATGGCGTTTGGATT | Start: 954 |
| Tm | 60.034 | 60.035 | End: 1110 |
| Size | 20 | 20 | 157 |
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