ssr_primer
Primer Details for TRINITY_DN86532_c3_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAACGTAGGAATCGCAGA | GAAGGTGTGAGGGCAGTGAA | Start: 312 |
| Tm | 59.755 | 59.891 | End: 545 |
| Size | 20 | 20 | 234 |
| Primer 2 details |
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| Sequence | GTGCAACGTAGGAATCGCAG | GAAGGTGTGAGGGCAGTGAA | Start: 311 |
| Tm | 59.63 | 59.891 | End: 545 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | TGCAACGTAGGAATCGCAGA | TTGAGGGAAGGGGAAGGTGT | Start: 312 |
| Tm | 59.755 | 60.403 | End: 557 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
|---|
| Sequence | GTGCAACGTAGGAATCGCAG | TTGAGGGAAGGGGAAGGTGT | Start: 311 |
| Tm | 59.63 | 60.403 | End: 557 |
| Size | 20 | 20 | 247 |
| Primer 5 details |
|---|
| Sequence | TGCAACGTAGGAATCGCAGA | AAGGTGTGAGGGCAGTGAAA | Start: 312 |
| Tm | 59.755 | 59.449 | End: 544 |
| Size | 20 | 20 | 233 |
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