ssr_primer
Primer Details for TRINITY_DN86530_c1_g1_i5 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACCTGTTCCTCGGTCGTT | GATGTTCGCGTCTTTCAGGC | Start: 1316 |
| Tm | 59.967 | 59.905 | End: 1594 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | ATTTCCTTGTAGTCCCGCCG | AACGACCGAGGAACAGGTTC | Start: 1236 |
| Tm | 60.108 | 59.967 | End: 1335 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | GAACCTGTTCCTCGGTCGTT | GAAGAATTGCGGTCGGAGGA | Start: 1316 |
| Tm | 59.967 | 60.108 | End: 1426 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | AGGCTGCATGGACTTGTTGA | AACGACCGAGGAACAGGTTC | Start: 1092 |
| Tm | 59.889 | 59.967 | End: 1335 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAAGGCTGCATGGACTTGT | AACGACCGAGGAACAGGTTC | Start: 1089 |
| Tm | 59.889 | 59.967 | End: 1335 |
| Size | 20 | 20 | 247 |
(G)10ataggaaaaaggagaagatgctcgtcgttcatttttaattcgcaaacgatcattccaaatgacaaattcgaatcccttgcactgc(A)24: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAACCTGTTCCTCGGTCGTT | GATGTTCGCGTCTTTCAGGC | Start: 1316 |
| Tm | 59.967 | 59.905 | End: 1594 |
| Size | 20 | 20 | 279 |
| Primer 2 details |
|---|
| Sequence | ATTTCCTTGTAGTCCCGCCG | AACGACCGAGGAACAGGTTC | Start: 1236 |
| Tm | 60.108 | 59.967 | End: 1335 |
| Size | 20 | 20 | 100 |
| Primer 3 details |
|---|
| Sequence | GAACCTGTTCCTCGGTCGTT | GAAGAATTGCGGTCGGAGGA | Start: 1316 |
| Tm | 59.967 | 60.108 | End: 1426 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | AGGCTGCATGGACTTGTTGA | AACGACCGAGGAACAGGTTC | Start: 1092 |
| Tm | 59.889 | 59.967 | End: 1335 |
| Size | 20 | 20 | 244 |
| Primer 5 details |
|---|
| Sequence | TGAAGGCTGCATGGACTTGT | AACGACCGAGGAACAGGTTC | Start: 1089 |
| Tm | 59.889 | 59.967 | End: 1335 |
| Size | 20 | 20 | 247 |
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