ssr_primer
Primer Details for TRINITY_DN86523_c2_g1_i1 |
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| (A)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGACACCCGAAAAAGAGCGT | TCGGGAGTGGTCATTTCTGC | Start: 99 |
| Tm | 59.894 | 60.036 | End: 330 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
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| Sequence | AGACACCCGAAAAAGAGCGT | GGGAGTGGTCATTTCTGCCA | Start: 99 |
| Tm | 59.894 | 59.962 | End: 328 |
| Size | 20 | 20 | 230 |
| Primer 3 details |
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| Sequence | GCCTGCAGGGTCGATTTTTG | TCGGGAGTGGTCATTTCTGC | Start: 140 |
| Tm | 60.109 | 60.036 | End: 330 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
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| Sequence | GCCTGCAGGGTCGATTTTTG | GGGAGTGGTCATTTCTGCCA | Start: 140 |
| Tm | 60.109 | 59.962 | End: 328 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
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| Sequence | ACACCCGAAAAAGAGCGTGA | TCGGGAGTGGTCATTTCTGC | Start: 101 |
| Tm | 60.179 | 60.036 | End: 330 |
| Size | 20 | 20 | 230 |
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