ssr_primer
Primer Details for TRINITY_DN86515_c1_g4_i1 |
|---|
| (G)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCTGATCCTTGCTACCCT | CCGAGCGGATTGGAACCATA | Start: 286 |
| Tm | 59.657 | 59.894 | End: 511 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TCGTATTCAGAACCCACGGC | AGGGTAGCAAGGATCAGGGA | Start: 119 |
| Tm | 60.109 | 59.657 | End: 305 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | TCCCTGATCCTTGCTACCCT | TGGAACCATAGTGCGTCGAC | Start: 286 |
| Tm | 59.657 | 60.109 | End: 501 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | TCCCTGATCCTTGCTACCCT | GGAACCATAGTGCGTCGACT | Start: 286 |
| Tm | 59.657 | 59.827 | End: 500 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
|---|
| Sequence | TCGTATTCAGAACCCACGGC | GAAAATTCTCAGGGCTGCGG | Start: 119 |
| Tm | 60.109 | 59.547 | End: 229 |
| Size | 20 | 20 | 111 |
(T)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCTGATCCTTGCTACCCT | CCGAGCGGATTGGAACCATA | Start: 286 |
| Tm | 59.657 | 59.894 | End: 511 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | TCGTATTCAGAACCCACGGC | AGGGTAGCAAGGATCAGGGA | Start: 119 |
| Tm | 60.109 | 59.657 | End: 305 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | TCCCTGATCCTTGCTACCCT | TGGAACCATAGTGCGTCGAC | Start: 286 |
| Tm | 59.657 | 60.109 | End: 501 |
| Size | 20 | 20 | 216 |
| Primer 4 details |
|---|
| Sequence | TCCCTGATCCTTGCTACCCT | GGAACCATAGTGCGTCGACT | Start: 286 |
| Tm | 59.657 | 59.827 | End: 500 |
| Size | 20 | 20 | 215 |
| Primer 5 details |
|---|
| Sequence | TCGTATTCAGAACCCACGGC | GAAAATTCTCAGGGCTGCGG | Start: 119 |
| Tm | 60.109 | 59.547 | End: 229 |
| Size | 20 | 20 | 111 |
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