ssr_primer
Primer Details for TRINITY_DN86498_c0_g1_i9 |
|---|
| (AT)6: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGCAAGTCATGGGGAGC | TCAGCGATCATCCTCTGTGC | Start: 286 |
| Tm | 60.035 | 59.896 | End: 388 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
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| Sequence | CTCAACCCGTCCCAGTGTAC | CCAACTGGGCTTTCATTCGC | Start: 758 |
| Tm | 60.038 | 60.109 | End: 858 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | ATCGTCAGGGCGTAGGATCT | GCTCCCCATGACTTGCTTCT | Start: 176 |
| Tm | 60.179 | 60.035 | End: 305 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | AGAAGCAAGTCATGGGGAGC | CAGCGATCATCCTCTGTGCA | Start: 286 |
| Tm | 60.035 | 60.179 | End: 387 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
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| Sequence | CCGAACCGAAGCTCCGTATT | TTGTCTGGCAGCATTCGAGT | Start: 1231 |
| Tm | 60.179 | 59.965 | End: 1373 |
| Size | 20 | 20 | 143 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGCAAGTCATGGGGAGC | TCAGCGATCATCCTCTGTGC | Start: 286 |
| Tm | 60.035 | 59.896 | End: 388 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | CTCAACCCGTCCCAGTGTAC | CCAACTGGGCTTTCATTCGC | Start: 758 |
| Tm | 60.038 | 60.109 | End: 858 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | ATCGTCAGGGCGTAGGATCT | GCTCCCCATGACTTGCTTCT | Start: 176 |
| Tm | 60.179 | 60.035 | End: 305 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | AGAAGCAAGTCATGGGGAGC | CAGCGATCATCCTCTGTGCA | Start: 286 |
| Tm | 60.035 | 60.179 | End: 387 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | CCGAACCGAAGCTCCGTATT | TTGTCTGGCAGCATTCGAGT | Start: 1231 |
| Tm | 60.179 | 59.965 | End: 1373 |
| Size | 20 | 20 | 143 |
(T)32: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAGCAAGTCATGGGGAGC | TCAGCGATCATCCTCTGTGC | Start: 286 |
| Tm | 60.035 | 59.896 | End: 388 |
| Size | 20 | 20 | 103 |
| Primer 2 details |
|---|
| Sequence | CTCAACCCGTCCCAGTGTAC | CCAACTGGGCTTTCATTCGC | Start: 758 |
| Tm | 60.038 | 60.109 | End: 858 |
| Size | 20 | 20 | 101 |
| Primer 3 details |
|---|
| Sequence | ATCGTCAGGGCGTAGGATCT | GCTCCCCATGACTTGCTTCT | Start: 176 |
| Tm | 60.179 | 60.035 | End: 305 |
| Size | 20 | 20 | 130 |
| Primer 4 details |
|---|
| Sequence | AGAAGCAAGTCATGGGGAGC | CAGCGATCATCCTCTGTGCA | Start: 286 |
| Tm | 60.035 | 60.179 | End: 387 |
| Size | 20 | 20 | 102 |
| Primer 5 details |
|---|
| Sequence | CCGAACCGAAGCTCCGTATT | TTGTCTGGCAGCATTCGAGT | Start: 1231 |
| Tm | 60.179 | 59.965 | End: 1373 |
| Size | 20 | 20 | 143 |
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