ssr_primer
Primer Details for TRINITY_DN86457_c3_g5_i1 |
|---|
| (TC)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATGGGGCCTCATTGAAGG | GCACCCACCCTAAAGCTTCT | Start: 833 |
| Tm | 60.033 | 59.962 | End: 997 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | ACATGGGGCCTCATTGAAGG | AGCACCCACCCTAAAGCTTC | Start: 833 |
| Tm | 60.033 | 59.962 | End: 998 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | ACATGGGGCCTCATTGAAGG | AAAAAGTCCTGCGCCAACAC | Start: 833 |
| Tm | 60.033 | 59.898 | End: 969 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | GCGGACAGGGCACATTATCT | CCTTCAATGAGGCCCCATGT | Start: 717 |
| Tm | 60.179 | 60.033 | End: 852 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGCCCATCGCTCATTTCACT | AGATAATGTGCCCTGTCCGC | Start: 520 |
| Tm | 60.035 | 60.179 | End: 736 |
| Size | 20 | 20 | 217 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACATGGGGCCTCATTGAAGG | GCACCCACCCTAAAGCTTCT | Start: 833 |
| Tm | 60.033 | 59.962 | End: 997 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | ACATGGGGCCTCATTGAAGG | AGCACCCACCCTAAAGCTTC | Start: 833 |
| Tm | 60.033 | 59.962 | End: 998 |
| Size | 20 | 20 | 166 |
| Primer 3 details |
|---|
| Sequence | ACATGGGGCCTCATTGAAGG | AAAAAGTCCTGCGCCAACAC | Start: 833 |
| Tm | 60.033 | 59.898 | End: 969 |
| Size | 20 | 20 | 137 |
| Primer 4 details |
|---|
| Sequence | GCGGACAGGGCACATTATCT | CCTTCAATGAGGCCCCATGT | Start: 717 |
| Tm | 60.179 | 60.033 | End: 852 |
| Size | 20 | 20 | 136 |
| Primer 5 details |
|---|
| Sequence | TGCCCATCGCTCATTTCACT | AGATAATGTGCCCTGTCCGC | Start: 520 |
| Tm | 60.035 | 60.179 | End: 736 |
| Size | 20 | 20 | 217 |
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