ssr_primer
Primer Details for TRINITY_DN86427_c4_g1_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAGTCTCCAGCCGTGTGA | AGGGGGCCCTTTATTTTCGG | Start: 146 |
| Tm | 59.966 | 60.033 | End: 272 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CCAGCCGTGTGAAATTCAGC | AGGGGGCCCTTTATTTTCGG | Start: 154 |
| Tm | 60.11 | 60.033 | End: 272 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CTGCCACACATGCGAAAGTC | AGGGGGCCCTTTATTTTCGG | Start: 133 |
| Tm | 60.11 | 60.033 | End: 272 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCCACACATGCGAAAGTCTC | AGGGGGCCCTTTATTTTCGG | Start: 135 |
| Tm | 59.833 | 60.033 | End: 272 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTTCTCTCAACGCCGCATTG | CAGATGGTCCAGGGGTAGGA | Start: 286 |
| Tm | 60.179 | 60.031 | End: 546 |
| Size | 20 | 20 | 261 |
(A)17: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAAAGTCTCCAGCCGTGTGA | AGGGGGCCCTTTATTTTCGG | Start: 146 |
| Tm | 59.966 | 60.033 | End: 272 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
|---|
| Sequence | CCAGCCGTGTGAAATTCAGC | AGGGGGCCCTTTATTTTCGG | Start: 154 |
| Tm | 60.11 | 60.033 | End: 272 |
| Size | 20 | 20 | 119 |
| Primer 3 details |
|---|
| Sequence | CTGCCACACATGCGAAAGTC | AGGGGGCCCTTTATTTTCGG | Start: 133 |
| Tm | 60.11 | 60.033 | End: 272 |
| Size | 20 | 20 | 140 |
| Primer 4 details |
|---|
| Sequence | GCCACACATGCGAAAGTCTC | AGGGGGCCCTTTATTTTCGG | Start: 135 |
| Tm | 59.833 | 60.033 | End: 272 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTTCTCTCAACGCCGCATTG | CAGATGGTCCAGGGGTAGGA | Start: 286 |
| Tm | 60.179 | 60.031 | End: 546 |
| Size | 20 | 20 | 261 |
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