ssr_primer
Primer Details for TRINITY_DN86325_c4_g1_i10 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCGTCAGCGCCTTCAAT | CCAGGCCCCTGCTAAATGAA | Start: 76 |
| Tm | 60.037 | 60.033 | End: 211 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
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| Sequence | TTCATTTAGCAGGGGCCTGG | TTTGCCACTGCGAATTCGTG | Start: 192 |
| Tm | 60.033 | 60.041 | End: 403 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
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| Sequence | GCTTATGCTCATCGTGTGCG | TTTGCCACTGCGAATTCGTG | Start: 148 |
| Tm | 60.043 | 60.041 | End: 403 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
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| Sequence | GCGTGCTTATGCTCATCGTG | TTTGCCACTGCGAATTCGTG | Start: 144 |
| Tm | 60.043 | 60.041 | End: 403 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
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| Sequence | AAGCAGCCGTCAGTACCTTC | GGGCTCTTCTCGTTGCGATA | Start: 766 |
| Tm | 60.037 | 59.898 | End: 997 |
| Size | 20 | 20 | 232 |
(A)67: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTTCGTCAGCGCCTTCAAT | CCAGGCCCCTGCTAAATGAA | Start: 76 |
| Tm | 60.037 | 60.033 | End: 211 |
| Size | 20 | 20 | 136 |
| Primer 2 details |
|---|
| Sequence | TTCATTTAGCAGGGGCCTGG | TTTGCCACTGCGAATTCGTG | Start: 192 |
| Tm | 60.033 | 60.041 | End: 403 |
| Size | 20 | 20 | 212 |
| Primer 3 details |
|---|
| Sequence | GCTTATGCTCATCGTGTGCG | TTTGCCACTGCGAATTCGTG | Start: 148 |
| Tm | 60.043 | 60.041 | End: 403 |
| Size | 20 | 20 | 256 |
| Primer 4 details |
|---|
| Sequence | GCGTGCTTATGCTCATCGTG | TTTGCCACTGCGAATTCGTG | Start: 144 |
| Tm | 60.043 | 60.041 | End: 403 |
| Size | 20 | 20 | 260 |
| Primer 5 details |
|---|
| Sequence | AAGCAGCCGTCAGTACCTTC | GGGCTCTTCTCGTTGCGATA | Start: 766 |
| Tm | 60.037 | 59.898 | End: 997 |
| Size | 20 | 20 | 232 |
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