ssr_primer
Primer Details for TRINITY_DN86318_c0_g5_i1 |
|---|
| (A)37: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGAGGAAACTGAGCAGGGC | CTTTGTTGCTGCCATCGTCC | Start: 89 |
| Tm | 60.035 | 60.11 | End: 353 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | GGACGATGGCAGCAACAAAG | AGCACACGTATTTACCGGCA | Start: 334 |
| Tm | 60.11 | 60.037 | End: 557 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | ATGAGGAAACTGAGCAGGGC | GTTGCTGCCATCGTCCAAAA | Start: 89 |
| Tm | 60.035 | 59.686 | End: 349 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | TTTTGGACGATGGCAGCAAC | AGCACACGTATTTACCGGCA | Start: 330 |
| Tm | 59.686 | 60.037 | End: 557 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | GAAACTGAGCAGGGCCCATA | CTTTGTTGCTGCCATCGTCC | Start: 94 |
| Tm | 59.744 | 60.11 | End: 353 |
| Size | 20 | 20 | 260 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGAGGAAACTGAGCAGGGC | CTTTGTTGCTGCCATCGTCC | Start: 89 |
| Tm | 60.035 | 60.11 | End: 353 |
| Size | 20 | 20 | 265 |
| Primer 2 details |
|---|
| Sequence | GGACGATGGCAGCAACAAAG | AGCACACGTATTTACCGGCA | Start: 334 |
| Tm | 60.11 | 60.037 | End: 557 |
| Size | 20 | 20 | 224 |
| Primer 3 details |
|---|
| Sequence | ATGAGGAAACTGAGCAGGGC | GTTGCTGCCATCGTCCAAAA | Start: 89 |
| Tm | 60.035 | 59.686 | End: 349 |
| Size | 20 | 20 | 261 |
| Primer 4 details |
|---|
| Sequence | TTTTGGACGATGGCAGCAAC | AGCACACGTATTTACCGGCA | Start: 330 |
| Tm | 59.686 | 60.037 | End: 557 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | GAAACTGAGCAGGGCCCATA | CTTTGTTGCTGCCATCGTCC | Start: 94 |
| Tm | 59.744 | 60.11 | End: 353 |
| Size | 20 | 20 | 260 |
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