ssr_primer
Primer Details for TRINITY_DN86309_c2_g1_i8 |
|---|
| (A)16: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGTCCTGTTGACTGCGA | GACTCGGTCTTGTGCTTCCA | Start: 1542 |
| Tm | 59.968 | 59.966 | End: 1656 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GACTGTCCTGTTGACTGCGA | TGACTCGGTCTTGTGCTTCC | Start: 1542 |
| Tm | 59.968 | 59.966 | End: 1657 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | GTGCCACTTTTGTAAGCGCA | GGGGCCACACAGAGAACTAC | Start: 645 |
| Tm | 59.97 | 60.036 | End: 893 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
|---|
| Sequence | AGACGAACGCGAACTCAACT | TTGGTTTTCCTACCCTCGCC | Start: 1263 |
| Tm | 59.97 | 59.963 | End: 1444 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | GACTGTCCTGTTGACTGCGA | GCAGACTGCTCCGTGTAGTT | Start: 1542 |
| Tm | 59.968 | 60.038 | End: 1772 |
| Size | 20 | 20 | 231 |
(A)10ttgataacgcagcgatcagtcttttgatatggcatacatatttattacttatgcatatacctaatgtgatgcatt(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GACTGTCCTGTTGACTGCGA | GACTCGGTCTTGTGCTTCCA | Start: 1542 |
| Tm | 59.968 | 59.966 | End: 1656 |
| Size | 20 | 20 | 115 |
| Primer 2 details |
|---|
| Sequence | GACTGTCCTGTTGACTGCGA | TGACTCGGTCTTGTGCTTCC | Start: 1542 |
| Tm | 59.968 | 59.966 | End: 1657 |
| Size | 20 | 20 | 116 |
| Primer 3 details |
|---|
| Sequence | GTGCCACTTTTGTAAGCGCA | GGGGCCACACAGAGAACTAC | Start: 645 |
| Tm | 59.97 | 60.036 | End: 893 |
| Size | 20 | 20 | 249 |
| Primer 4 details |
|---|
| Sequence | AGACGAACGCGAACTCAACT | TTGGTTTTCCTACCCTCGCC | Start: 1263 |
| Tm | 59.97 | 59.963 | End: 1444 |
| Size | 20 | 20 | 182 |
| Primer 5 details |
|---|
| Sequence | GACTGTCCTGTTGACTGCGA | GCAGACTGCTCCGTGTAGTT | Start: 1542 |
| Tm | 59.968 | 60.038 | End: 1772 |
| Size | 20 | 20 | 231 |
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