ssr_primer
Primer Details for TRINITY_DN86308_c1_g1_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGAGGCAGTGAACAGCT | TCAGACGAAACCTTCCTGCC | Start: 267 |
| Tm | 59.964 | 59.965 | End: 528 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | GGAAGAGGCAGTGAACAGCT | ACGAAACCTTCCTGCCCAAA | Start: 267 |
| Tm | 59.964 | 60.106 | End: 524 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | AACACCGGAAATTGGAGGCT | AGCTGTTCACTGCCTCTTCC | Start: 134 |
| Tm | 59.888 | 59.964 | End: 286 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | ATTCCCCCGAGAGCAACAAG | AGGGAGTAACTGAAGGGCCT | Start: 3310 |
| Tm | 60.035 | 59.881 | End: 3413 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | CCCCCGCTACATTACCCAAG | AGCTGTTCACTGCCTCTTCC | Start: 28 |
| Tm | 60.179 | 59.964 | End: 286 |
| Size | 20 | 20 | 259 |
(C)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGAAGAGGCAGTGAACAGCT | TCAGACGAAACCTTCCTGCC | Start: 267 |
| Tm | 59.964 | 59.965 | End: 528 |
| Size | 20 | 20 | 262 |
| Primer 2 details |
|---|
| Sequence | GGAAGAGGCAGTGAACAGCT | ACGAAACCTTCCTGCCCAAA | Start: 267 |
| Tm | 59.964 | 60.106 | End: 524 |
| Size | 20 | 20 | 258 |
| Primer 3 details |
|---|
| Sequence | AACACCGGAAATTGGAGGCT | AGCTGTTCACTGCCTCTTCC | Start: 134 |
| Tm | 59.888 | 59.964 | End: 286 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | ATTCCCCCGAGAGCAACAAG | AGGGAGTAACTGAAGGGCCT | Start: 3310 |
| Tm | 60.035 | 59.881 | End: 3413 |
| Size | 20 | 20 | 104 |
| Primer 5 details |
|---|
| Sequence | CCCCCGCTACATTACCCAAG | AGCTGTTCACTGCCTCTTCC | Start: 28 |
| Tm | 60.179 | 59.964 | End: 286 |
| Size | 20 | 20 | 259 |
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