ssr_primer
Primer Details for TRINITY_DN86303_c3_g1_i3 |
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| (TC)7ctttctttttttttc(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCGGCATGAAGTTCGTT | AACTGAGCATTGCAACAGCG | Start: 587 |
| Tm | 59.965 | 60.04 | End: 738 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
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| Sequence | ACGATGGCAGTGATGAAGCA | AACTGAGCATTGCAACAGCG | Start: 637 |
| Tm | 60.036 | 60.04 | End: 738 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
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| Sequence | CGCAGATTTCCATCGCTGTG | AGCGATCGATTTTTGCAGGC | Start: 264 |
| Tm | 59.973 | 59.901 | End: 491 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
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| Sequence | GCCTGCAAAAATCGATCGCT | AACGAACTTCATGCCGGAGT | Start: 472 |
| Tm | 59.901 | 59.965 | End: 606 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
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| Sequence | GCAATGACGACCATCACGTG | AACTGAGCATTGCAACAGCG | Start: 560 |
| Tm | 59.905 | 60.04 | End: 738 |
| Size | 20 | 20 | 179 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCGGCATGAAGTTCGTT | AACTGAGCATTGCAACAGCG | Start: 587 |
| Tm | 59.965 | 60.04 | End: 738 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | ACGATGGCAGTGATGAAGCA | AACTGAGCATTGCAACAGCG | Start: 637 |
| Tm | 60.036 | 60.04 | End: 738 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | CGCAGATTTCCATCGCTGTG | AGCGATCGATTTTTGCAGGC | Start: 264 |
| Tm | 59.973 | 59.901 | End: 491 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | GCCTGCAAAAATCGATCGCT | AACGAACTTCATGCCGGAGT | Start: 472 |
| Tm | 59.901 | 59.965 | End: 606 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GCAATGACGACCATCACGTG | AACTGAGCATTGCAACAGCG | Start: 560 |
| Tm | 59.905 | 60.04 | End: 738 |
| Size | 20 | 20 | 179 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCCGGCATGAAGTTCGTT | AACTGAGCATTGCAACAGCG | Start: 587 |
| Tm | 59.965 | 60.04 | End: 738 |
| Size | 20 | 20 | 152 |
| Primer 2 details |
|---|
| Sequence | ACGATGGCAGTGATGAAGCA | AACTGAGCATTGCAACAGCG | Start: 637 |
| Tm | 60.036 | 60.04 | End: 738 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | CGCAGATTTCCATCGCTGTG | AGCGATCGATTTTTGCAGGC | Start: 264 |
| Tm | 59.973 | 59.901 | End: 491 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | GCCTGCAAAAATCGATCGCT | AACGAACTTCATGCCGGAGT | Start: 472 |
| Tm | 59.901 | 59.965 | End: 606 |
| Size | 20 | 20 | 135 |
| Primer 5 details |
|---|
| Sequence | GCAATGACGACCATCACGTG | AACTGAGCATTGCAACAGCG | Start: 560 |
| Tm | 59.905 | 60.04 | End: 738 |
| Size | 20 | 20 | 179 |
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