ssr_primer
Primer Details for TRINITY_DN86262_c7_g5_i3 |
|---|
| (A)18taggtttaaaaagg(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTACCCTTTCAGCATGCT | GCTGCTGACGAAAAACCTCG | Start: 437 |
| Tm | 59.302 | 60.111 | End: 667 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TCAGTTTGGTCAGACGCTGT | AGCGCATCAATCCTGACTGA | Start: 603 |
| Tm | 59.536 | 59.461 | End: 741 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TGGTACCCTTTCAGCATGCT | ATTGTGCACAACAGAGCTGC | Start: 437 |
| Tm | 59.302 | 59.687 | End: 682 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
|---|
| Sequence | TTCGTCAGCAGCTCTGTTGT | ACGGCACACTCCTTCCAATA | Start: 656 |
| Tm | 59.895 | 59.019 | End: 779 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TTCGTCAGCAGCTCTGTTGT | TACGGCACACTCCTTCCAAT | Start: 656 |
| Tm | 59.895 | 59.019 | End: 780 |
| Size | 20 | 20 | 125 |
(T)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGTACCCTTTCAGCATGCT | GCTGCTGACGAAAAACCTCG | Start: 437 |
| Tm | 59.302 | 60.111 | End: 667 |
| Size | 20 | 20 | 231 |
| Primer 2 details |
|---|
| Sequence | TCAGTTTGGTCAGACGCTGT | AGCGCATCAATCCTGACTGA | Start: 603 |
| Tm | 59.536 | 59.461 | End: 741 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
|---|
| Sequence | TGGTACCCTTTCAGCATGCT | ATTGTGCACAACAGAGCTGC | Start: 437 |
| Tm | 59.302 | 59.687 | End: 682 |
| Size | 20 | 20 | 246 |
| Primer 4 details |
|---|
| Sequence | TTCGTCAGCAGCTCTGTTGT | ACGGCACACTCCTTCCAATA | Start: 656 |
| Tm | 59.895 | 59.019 | End: 779 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | TTCGTCAGCAGCTCTGTTGT | TACGGCACACTCCTTCCAAT | Start: 656 |
| Tm | 59.895 | 59.019 | End: 780 |
| Size | 20 | 20 | 125 |
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