ssr_primer
Primer Details for TRINITY_DN86253_c1_g1_i8 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGTGAGCAGAGCAAGGAT | GATGCAGAGGTTTTGCGCAA | Start: 2252 |
| Tm | 60.036 | 60.039 | End: 2454 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TGCCAAGTCCAGCAATAGCA | GATGCAGAGGTTTTGCGCAA | Start: 2199 |
| Tm | 59.962 | 60.039 | End: 2454 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TGCTCCTGACAACTTTGGCA | CAATACGCCGTCATTGCAGG | Start: 1770 |
| Tm | 60.107 | 59.972 | End: 1875 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TGCTCCTGACAACTTTGGCA | CCAATACGCCGTCATTGCAG | Start: 1770 |
| Tm | 60.107 | 59.972 | End: 1876 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | GGTGTGAGCAGAGCAAGGAT | CTGCCAACCCATTTGATGCC | Start: 2252 |
| Tm | 60.036 | 60.108 | End: 2480 |
| Size | 20 | 20 | 229 |
(A)10ttttcatgctaaaatagagagaatagaaatgttaataaaatattgaaggagtcaacaagtaaaattaaacaattcaaaattcgat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGTGTGAGCAGAGCAAGGAT | GATGCAGAGGTTTTGCGCAA | Start: 2252 |
| Tm | 60.036 | 60.039 | End: 2454 |
| Size | 20 | 20 | 203 |
| Primer 2 details |
|---|
| Sequence | TGCCAAGTCCAGCAATAGCA | GATGCAGAGGTTTTGCGCAA | Start: 2199 |
| Tm | 59.962 | 60.039 | End: 2454 |
| Size | 20 | 20 | 256 |
| Primer 3 details |
|---|
| Sequence | TGCTCCTGACAACTTTGGCA | CAATACGCCGTCATTGCAGG | Start: 1770 |
| Tm | 60.107 | 59.972 | End: 1875 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | TGCTCCTGACAACTTTGGCA | CCAATACGCCGTCATTGCAG | Start: 1770 |
| Tm | 60.107 | 59.972 | End: 1876 |
| Size | 20 | 20 | 107 |
| Primer 5 details |
|---|
| Sequence | GGTGTGAGCAGAGCAAGGAT | CTGCCAACCCATTTGATGCC | Start: 2252 |
| Tm | 60.036 | 60.108 | End: 2480 |
| Size | 20 | 20 | 229 |
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