ssr_primer
Primer Details for TRINITY_DN86252_c0_g1_i5 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTGACTGATCCGCACTT | TGGAGGAAGTTTGGCGACTC | Start: 3398 |
| Tm | 59.965 | 59.965 | End: 3543 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | GAGTCGCCAAACTTCCTCCA | TGAACCTTCAGACGTTGCCA | Start: 3524 |
| Tm | 59.965 | 59.821 | End: 3713 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCTGCCGTCCATACCAAAAC | AAGCAAAACCGCCACAACTC | Start: 1218 |
| Tm | 59.83 | 59.898 | End: 1496 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | GCTGCCGTCCATACCAAAAC | AACCGCCACAACTCCATTCT | Start: 1218 |
| Tm | 59.83 | 59.89 | End: 1490 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | TGCTGCCGTCCATACCAAAA | AAGCAAAACCGCCACAACTC | Start: 1217 |
| Tm | 60.251 | 59.898 | End: 1496 |
| Size | 20 | 20 | 280 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTGACTGATCCGCACTT | TGGAGGAAGTTTGGCGACTC | Start: 3398 |
| Tm | 59.965 | 59.965 | End: 3543 |
| Size | 20 | 20 | 146 |
| Primer 2 details |
|---|
| Sequence | GAGTCGCCAAACTTCCTCCA | TGAACCTTCAGACGTTGCCA | Start: 3524 |
| Tm | 59.965 | 59.821 | End: 3713 |
| Size | 20 | 20 | 190 |
| Primer 3 details |
|---|
| Sequence | GCTGCCGTCCATACCAAAAC | AAGCAAAACCGCCACAACTC | Start: 1218 |
| Tm | 59.83 | 59.898 | End: 1496 |
| Size | 20 | 20 | 279 |
| Primer 4 details |
|---|
| Sequence | GCTGCCGTCCATACCAAAAC | AACCGCCACAACTCCATTCT | Start: 1218 |
| Tm | 59.83 | 59.89 | End: 1490 |
| Size | 20 | 20 | 273 |
| Primer 5 details |
|---|
| Sequence | TGCTGCCGTCCATACCAAAA | AAGCAAAACCGCCACAACTC | Start: 1217 |
| Tm | 60.251 | 59.898 | End: 1496 |
| Size | 20 | 20 | 280 |
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