ssr_primer
Primer Details for TRINITY_DN86230_c5_g1_i1 |
|---|
| (C)27aaaac(A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTGTTCCACGGAGCAAC | GACTTTCGGCTCCCAACTGA | Start: 1451 |
| Tm | 59.971 | 59.965 | End: 1656 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | GTTGTGTTCCACGGAGCAAC | CTGACTTTCGGCTCCCAACT | Start: 1451 |
| Tm | 59.971 | 59.965 | End: 1658 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GTTGTGTTCCACGGAGCAAC | GCGATGGTGTCTCCTGAACA | Start: 1451 |
| Tm | 59.971 | 60.037 | End: 1623 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TCGGGTACACCAAACTTCCG | GGTTAAGGTTGCTCCGTGGA | Start: 1217 |
| Tm | 59.966 | 59.964 | End: 1477 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TCGGGTACACCAAACTTCCG | AGGTTAAGGTTGCTCCGTGG | Start: 1217 |
| Tm | 59.966 | 59.964 | End: 1478 |
| Size | 20 | 20 | 262 |
(T)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGTGTTCCACGGAGCAAC | GACTTTCGGCTCCCAACTGA | Start: 1451 |
| Tm | 59.971 | 59.965 | End: 1656 |
| Size | 20 | 20 | 206 |
| Primer 2 details |
|---|
| Sequence | GTTGTGTTCCACGGAGCAAC | CTGACTTTCGGCTCCCAACT | Start: 1451 |
| Tm | 59.971 | 59.965 | End: 1658 |
| Size | 20 | 20 | 208 |
| Primer 3 details |
|---|
| Sequence | GTTGTGTTCCACGGAGCAAC | GCGATGGTGTCTCCTGAACA | Start: 1451 |
| Tm | 59.971 | 60.037 | End: 1623 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TCGGGTACACCAAACTTCCG | GGTTAAGGTTGCTCCGTGGA | Start: 1217 |
| Tm | 59.966 | 59.964 | End: 1477 |
| Size | 20 | 20 | 261 |
| Primer 5 details |
|---|
| Sequence | TCGGGTACACCAAACTTCCG | AGGTTAAGGTTGCTCCGTGG | Start: 1217 |
| Tm | 59.966 | 59.964 | End: 1478 |
| Size | 20 | 20 | 262 |
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