ssr_primer
Primer Details for TRINITY_DN86149_c3_g1_i2 |
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| (T)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAGCTCTTGCATGGGTCAG | AGTTCGACTGGATGCAGTGG | Start: 990 |
| Tm | 60.036 | 60.037 | End: 1180 |
| Size | 20 | 20 | 191 |
| Primer 2 details |
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| Sequence | CGATCTGGCTTAGACGTCCC | TGATTCCTTTGTGGCCAGCA | Start: 464 |
| Tm | 59.969 | 60.179 | End: 611 |
| Size | 20 | 20 | 148 |
| Primer 3 details |
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| Sequence | TCTGGCTTAGACGTCCCTGA | TGATTCCTTTGTGGCCAGCA | Start: 467 |
| Tm | 59.961 | 60.179 | End: 611 |
| Size | 20 | 20 | 145 |
| Primer 4 details |
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| Sequence | CGATCTGGCTTAGACGTCCC | GATTCCTTTGTGGCCAGCAC | Start: 464 |
| Tm | 59.969 | 59.755 | End: 610 |
| Size | 20 | 20 | 147 |
| Primer 5 details |
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| Sequence | GCTTAGACGTCCCTGAGCTC | TGATTCCTTTGTGGCCAGCA | Start: 471 |
| Tm | 59.898 | 60.179 | End: 611 |
| Size | 20 | 20 | 141 |
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