ssr_primer
Primer Details for TRINITY_DN86146_c0_g1_i6 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTATTCGAGCCGCAACTGC | CACTCACACACAGCTGGGAT | Start: 2978 |
| Tm | 59.973 | 59.964 | End: 3126 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CTTATTCGAGCCGCAACTGC | CCATCCACCTCCACTCACAC | Start: 2978 |
| Tm | 59.973 | 60.036 | End: 3137 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CACTCACACACAGCTGGGAT | Start: 2899 |
| Tm | 59.966 | 59.964 | End: 3126 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CCATCCACCTCCACTCACAC | Start: 2899 |
| Tm | 59.966 | 60.036 | End: 3137 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | GTTCCTGCTTCCTGACCCTC | CACTCACACACAGCTGGGAT | Start: 2903 |
| Tm | 60.036 | 59.964 | End: 3126 |
| Size | 20 | 20 | 224 |
(GTA)5atgtaaactgcaacaacagtagtaataattctaattctagcaa(TAG)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTATTCGAGCCGCAACTGC | CACTCACACACAGCTGGGAT | Start: 2978 |
| Tm | 59.973 | 59.964 | End: 3126 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CTTATTCGAGCCGCAACTGC | CCATCCACCTCCACTCACAC | Start: 2978 |
| Tm | 59.973 | 60.036 | End: 3137 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CACTCACACACAGCTGGGAT | Start: 2899 |
| Tm | 59.966 | 59.964 | End: 3126 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CCATCCACCTCCACTCACAC | Start: 2899 |
| Tm | 59.966 | 60.036 | End: 3137 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | GTTCCTGCTTCCTGACCCTC | CACTCACACACAGCTGGGAT | Start: 2903 |
| Tm | 60.036 | 59.964 | End: 3126 |
| Size | 20 | 20 | 224 |
(A)10tatgggaaaatgggttagaat(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CTTATTCGAGCCGCAACTGC | CACTCACACACAGCTGGGAT | Start: 2978 |
| Tm | 59.973 | 59.964 | End: 3126 |
| Size | 20 | 20 | 149 |
| Primer 2 details |
|---|
| Sequence | CTTATTCGAGCCGCAACTGC | CCATCCACCTCCACTCACAC | Start: 2978 |
| Tm | 59.973 | 60.036 | End: 3137 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CACTCACACACAGCTGGGAT | Start: 2899 |
| Tm | 59.966 | 59.964 | End: 3126 |
| Size | 20 | 20 | 228 |
| Primer 4 details |
|---|
| Sequence | TCGTGTTCCTGCTTCCTGAC | CCATCCACCTCCACTCACAC | Start: 2899 |
| Tm | 59.966 | 60.036 | End: 3137 |
| Size | 20 | 20 | 239 |
| Primer 5 details |
|---|
| Sequence | GTTCCTGCTTCCTGACCCTC | CACTCACACACAGCTGGGAT | Start: 2903 |
| Tm | 60.036 | 59.964 | End: 3126 |
| Size | 20 | 20 | 224 |
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