ssr_primer
Primer Details for TRINITY_DN86036_c5_g2_i6 |
|---|
| (T)33: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTCCTCCGGTGTTGCTT | CTCAGGAAGCCAAGCCGTAT | Start: 1169 |
| Tm | 59.89 | 59.821 | End: 1292 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AGAGCAGCCTTTGGGTCATC | GTCTACGTCGGTTGTGCAGT | Start: 2030 |
| Tm | 60.035 | 60.318 | End: 2271 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
|---|
| Sequence | CCGGTGTTGCTTGATGATGC | CTCAGGAAGCCAAGCCGTAT | Start: 1177 |
| Tm | 60.179 | 59.821 | End: 1292 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | CCTGCTACTAGCTTTCGCCA | GCAAGCTCACAGAGTCGAGA | Start: 74 |
| Tm | 59.824 | 59.758 | End: 296 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | ACCTGCTACTAGCTTTCGCC | GCAAGCTCACAGAGTCGAGA | Start: 73 |
| Tm | 59.824 | 59.758 | End: 296 |
| Size | 20 | 20 | 224 |
(AAT)5gagtattcaatcaacaaattactgcacaaccgacgtagacgtaaagcttccccggcacctttgcaaattctcttattaaaacagcgtaagtattca(T)16: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGTTCCTCCGGTGTTGCTT | CTCAGGAAGCCAAGCCGTAT | Start: 1169 |
| Tm | 59.89 | 59.821 | End: 1292 |
| Size | 20 | 20 | 124 |
| Primer 2 details |
|---|
| Sequence | AGAGCAGCCTTTGGGTCATC | GTCTACGTCGGTTGTGCAGT | Start: 2030 |
| Tm | 60.035 | 60.318 | End: 2271 |
| Size | 20 | 20 | 242 |
| Primer 3 details |
|---|
| Sequence | CCGGTGTTGCTTGATGATGC | CTCAGGAAGCCAAGCCGTAT | Start: 1177 |
| Tm | 60.179 | 59.821 | End: 1292 |
| Size | 20 | 20 | 116 |
| Primer 4 details |
|---|
| Sequence | CCTGCTACTAGCTTTCGCCA | GCAAGCTCACAGAGTCGAGA | Start: 74 |
| Tm | 59.824 | 59.758 | End: 296 |
| Size | 20 | 20 | 223 |
| Primer 5 details |
|---|
| Sequence | ACCTGCTACTAGCTTTCGCC | GCAAGCTCACAGAGTCGAGA | Start: 73 |
| Tm | 59.824 | 59.758 | End: 296 |
| Size | 20 | 20 | 224 |
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