ssr_primer
Primer Details for TRINITY_DN86023_c1_g3_i1 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGTCGTTTTTGGACGTCA | ACTGAATGTTGCCGTTGTGC | Start: 976 |
| Tm | 60.108 | 59.969 | End: 1149 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | CACGCACACAAGGGACTAGT | ATAATACCAGCCTGCCCTGC | Start: 92 |
| Tm | 59.966 | 59.889 | End: 322 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTCCTTTGCACGCACACAAG | ATAATACCAGCCTGCCCTGC | Start: 84 |
| Tm | 59.9 | 59.889 | End: 322 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CTTCCTTTGCACGCACACAA | ATAATACCAGCCTGCCCTGC | Start: 83 |
| Tm | 59.9 | 59.889 | End: 322 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | GGGTCGTTTTTGGACGTCAC | ACTGAATGTTGCCGTTGTGC | Start: 977 |
| Tm | 59.694 | 59.969 | End: 1149 |
| Size | 20 | 20 | 173 |
(T)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGTCGTTTTTGGACGTCA | ACTGAATGTTGCCGTTGTGC | Start: 976 |
| Tm | 60.108 | 59.969 | End: 1149 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | CACGCACACAAGGGACTAGT | ATAATACCAGCCTGCCCTGC | Start: 92 |
| Tm | 59.966 | 59.889 | End: 322 |
| Size | 20 | 20 | 231 |
| Primer 3 details |
|---|
| Sequence | TTCCTTTGCACGCACACAAG | ATAATACCAGCCTGCCCTGC | Start: 84 |
| Tm | 59.9 | 59.889 | End: 322 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
|---|
| Sequence | CTTCCTTTGCACGCACACAA | ATAATACCAGCCTGCCCTGC | Start: 83 |
| Tm | 59.9 | 59.889 | End: 322 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
|---|
| Sequence | GGGTCGTTTTTGGACGTCAC | ACTGAATGTTGCCGTTGTGC | Start: 977 |
| Tm | 59.694 | 59.969 | End: 1149 |
| Size | 20 | 20 | 173 |
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