ssr_primer
Primer Details for TRINITY_DN86018_c1_g1_i3 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCACTCATGGGATGGCAA | CAACAGCAAGGAGGGATGGT | Start: 786 |
| Tm | 59.96 | 59.961 | End: 948 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | AAGCACTCATGGGATGGCAA | CAGCAAGGAGGGATGGTGAG | Start: 786 |
| Tm | 59.96 | 60.107 | End: 945 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | AGCACTCATGGGATGGCAAT | CAACAGCAAGGAGGGATGGT | Start: 787 |
| Tm | 59.739 | 59.961 | End: 948 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTTTTGAAGCAGGTACGCGC | TGTCAACATTAGCAGCCCCC | Start: 177 |
| Tm | 60.041 | 60.323 | End: 300 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CATTTTGTATCCTCGCGCCG | TGTCAACATTAGCAGCCCCC | Start: 156 |
| Tm | 60.042 | 60.323 | End: 300 |
| Size | 20 | 20 | 145 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCACTCATGGGATGGCAA | CAACAGCAAGGAGGGATGGT | Start: 786 |
| Tm | 59.96 | 59.961 | End: 948 |
| Size | 20 | 20 | 163 |
| Primer 2 details |
|---|
| Sequence | AAGCACTCATGGGATGGCAA | CAGCAAGGAGGGATGGTGAG | Start: 786 |
| Tm | 59.96 | 60.107 | End: 945 |
| Size | 20 | 20 | 160 |
| Primer 3 details |
|---|
| Sequence | AGCACTCATGGGATGGCAAT | CAACAGCAAGGAGGGATGGT | Start: 787 |
| Tm | 59.739 | 59.961 | End: 948 |
| Size | 20 | 20 | 162 |
| Primer 4 details |
|---|
| Sequence | TTTTTGAAGCAGGTACGCGC | TGTCAACATTAGCAGCCCCC | Start: 177 |
| Tm | 60.041 | 60.323 | End: 300 |
| Size | 20 | 20 | 124 |
| Primer 5 details |
|---|
| Sequence | CATTTTGTATCCTCGCGCCG | TGTCAACATTAGCAGCCCCC | Start: 156 |
| Tm | 60.042 | 60.323 | End: 300 |
| Size | 20 | 20 | 145 |
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