ssr_primer
Primer Details for TRINITY_DN85975_c3_g1_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTGCGAGGCGTGAATGATC | GCCGCCGGATAATCCAAAAC | Start: 9 |
| Tm | 59.973 | 59.97 | End: 185 |
| Size | 20 | 20 | 177 |
| Primer 2 details |
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| Sequence | GTTGCGAGGCGTGAATGATC | CGGCTCTCTCCGCGATAAAT | Start: 9 |
| Tm | 59.973 | 60.039 | End: 166 |
| Size | 20 | 20 | 158 |
| Primer 3 details |
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| Sequence | GTTTTGGATTATCCGGCGGC | CGCACGGAACTCCACATACT | Start: 166 |
| Tm | 59.97 | 60.109 | End: 435 |
| Size | 20 | 20 | 270 |
| Primer 4 details |
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| Sequence | GTTTTGGATTATCCGGCGGC | AACAAAGCATCTCTTCGCGC | Start: 166 |
| Tm | 59.97 | 59.833 | End: 368 |
| Size | 20 | 20 | 203 |
| Primer 5 details |
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| Sequence | CGGAGAGAGCCGTTTTGGAT | AACAAAGCATCTCTTCGCGC | Start: 155 |
| Tm | 60.108 | 59.833 | End: 368 |
| Size | 20 | 20 | 214 |
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