ssr_primer
Primer Details for TRINITY_DN85962_c2_g1_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCACACGGACGATGCTTCA | TGGCGTCACATCTAATCGGG | Start: 140 |
| Tm | 59.967 | 59.896 | End: 252 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | GTGGGCAAACAACGTGGTTT | Start: 140 |
| Tm | 59.967 | 60.109 | End: 308 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | ACCCCTTTCCACCTGCTTTC | Start: 140 |
| Tm | 59.967 | 60.179 | End: 394 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | TGGGAAACTGAGTCCTGCAC | GTGGGCAAACAACGTGGTTT | Start: 179 |
| Tm | 59.891 | 60.109 | End: 308 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | GTGAGTGGGCAAACAACGTG | Start: 140 |
| Tm | 59.967 | 60.248 | End: 312 |
| Size | 20 | 20 | 173 |
(C)14agtcaagggagaatcattgcgagggggggaaagcaggtggaaaggggtaagtgtgatcttcaatccttggggattaaatcgaggaaaat(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTCACACGGACGATGCTTCA | TGGCGTCACATCTAATCGGG | Start: 140 |
| Tm | 59.967 | 59.896 | End: 252 |
| Size | 20 | 20 | 113 |
| Primer 2 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | GTGGGCAAACAACGTGGTTT | Start: 140 |
| Tm | 59.967 | 60.109 | End: 308 |
| Size | 20 | 20 | 169 |
| Primer 3 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | ACCCCTTTCCACCTGCTTTC | Start: 140 |
| Tm | 59.967 | 60.179 | End: 394 |
| Size | 20 | 20 | 255 |
| Primer 4 details |
|---|
| Sequence | TGGGAAACTGAGTCCTGCAC | GTGGGCAAACAACGTGGTTT | Start: 179 |
| Tm | 59.891 | 60.109 | End: 308 |
| Size | 20 | 20 | 130 |
| Primer 5 details |
|---|
| Sequence | TTCACACGGACGATGCTTCA | GTGAGTGGGCAAACAACGTG | Start: 140 |
| Tm | 59.967 | 60.248 | End: 312 |
| Size | 20 | 20 | 173 |
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