ssr_primer
Primer Details for TRINITY_DN85960_c3_g1_i7 |
|---|
| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTGTCTACCGAGGTCT | TTCTTCATGGCGTCCAACGA | Start: 249 |
| Tm | 60.033 | 59.966 | End: 413 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | TCGTTGGACGCCATGAAGAA | TGCATAGTAAGCGCACCCAA | Start: 394 |
| Tm | 59.966 | 60.036 | End: 569 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | CATGGCTGTCTACCGAGGTC | TTCTTCATGGCGTCCAACGA | Start: 248 |
| Tm | 59.897 | 59.966 | End: 413 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
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| Sequence | CCAGCAGGATTCCTAGCAGG | GAAAAAGTTGCCCCGGACAC | Start: 801 |
| Tm | 59.892 | 59.968 | End: 1065 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TTGGGTGCGCTTACTATGCA | CCTGCTAGGAATCCTGCTGG | Start: 550 |
| Tm | 60.036 | 59.892 | End: 820 |
| Size | 20 | 20 | 271 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ATGGCTGTCTACCGAGGTCT | TTCTTCATGGCGTCCAACGA | Start: 249 |
| Tm | 60.033 | 59.966 | End: 413 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
|---|
| Sequence | TCGTTGGACGCCATGAAGAA | TGCATAGTAAGCGCACCCAA | Start: 394 |
| Tm | 59.966 | 60.036 | End: 569 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | CATGGCTGTCTACCGAGGTC | TTCTTCATGGCGTCCAACGA | Start: 248 |
| Tm | 59.897 | 59.966 | End: 413 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
|---|
| Sequence | CCAGCAGGATTCCTAGCAGG | GAAAAAGTTGCCCCGGACAC | Start: 801 |
| Tm | 59.892 | 59.968 | End: 1065 |
| Size | 20 | 20 | 265 |
| Primer 5 details |
|---|
| Sequence | TTGGGTGCGCTTACTATGCA | CCTGCTAGGAATCCTGCTGG | Start: 550 |
| Tm | 60.036 | 59.892 | End: 820 |
| Size | 20 | 20 | 271 |
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