Primer Details for TRINITY_DN85953_c9_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACTCAAATCGCAAATCCCTGC | TGGTGTTTATATGACAGGTTTTACGG | Start: 67 |
| Tm | 59.526 | 59.628 | End: 168 |
| Size | 21 | 26 | 102 |
| Primer 2 details |
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| Sequence | ACTCAAATCGCAAATCCCTGC | GGTGTTTATATGACAGGTTTTACGGA | Start: 67 |
| Tm | 59.526 | 59.405 | End: 167 |
| Size | 21 | 26 | 101 |
| Primer 3 details |
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| Sequence | ACTCAAATCGCAAATCCCTGC | GGTGTTTATATGACAGGTTTTACGG | Start: 67 |
| Tm | 59.526 | 58.222 | End: 167 |
| Size | 21 | 25 | 101 |
| Primer 4 details |
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| Sequence | ACTCAAATCGCAAATCCCTGC | TTGGTGTTTATATGACAGGTTTTACGG | Start: 67 |
| Tm | 59.526 | 60.095 | End: 169 |
| Size | 21 | 27 | 103 |
| Primer 5 details |
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| Sequence | CTCAAATCGCAAATCCCTGC | TGGTGTTTATATGACAGGTTTTACGG | Start: 68 |
| Tm | 57.801 | 59.628 | End: 168 |
| Size | 20 | 26 | 101 |
