ssr_primer
Primer Details for TRINITY_DN85949_c2_g1_i14 |
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| (T)33: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTAACTGTGCTCACGGGTGA | TCGTACCCGAGCTCTGTGTA | Start: 2578 |
| Tm | 59.967 | 60.036 | End: 2830 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | AGTAACTGTGCTCACGGGTG | TCGTACCCGAGCTCTGTGTA | Start: 2577 |
| Tm | 59.966 | 60.036 | End: 2830 |
| Size | 20 | 20 | 254 |
| Primer 3 details |
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| Sequence | TGGGAGCCTAGACTTGACGA | GGAGTGGAAAACGGCCAAAC | Start: 2394 |
| Tm | 59.961 | 59.968 | End: 2553 |
| Size | 20 | 20 | 160 |
| Primer 4 details |
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| Sequence | CCCGGGCCAAAGTCATACTT | TTGGTTTTGGGCGCAACAAA | Start: 706 |
| Tm | 60.034 | 60.036 | End: 862 |
| Size | 20 | 20 | 157 |
| Primer 5 details |
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| Sequence | CCCGGGCCAAAGTCATACTT | TTTGGTTTTGGGCGCAACAA | Start: 706 |
| Tm | 60.034 | 60.036 | End: 863 |
| Size | 20 | 20 | 158 |
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