ssr_primer
Primer Details for TRINITY_DN85938_c1_g1_i1 |
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| (A)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTAGAGGAGGGGCCACTGTA | AGCTTCTTGCAGGGCTTTCA | Start: 1277 |
| Tm | 60.032 | 60.179 | End: 1459 |
| Size | 20 | 20 | 183 |
| Primer 2 details |
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| Sequence | TTACAATGGTCCCACTCCGC | AGCTTCTTGCAGGGCTTTCA | Start: 1321 |
| Tm | 60.036 | 60.179 | End: 1459 |
| Size | 20 | 20 | 139 |
| Primer 3 details |
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| Sequence | AGGAGGGGCCACTGTATTCT | AGCTTCTTGCAGGGCTTTCA | Start: 1281 |
| Tm | 59.955 | 60.179 | End: 1459 |
| Size | 20 | 20 | 179 |
| Primer 4 details |
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| Sequence | AGAGGAGGGGCCACTGTATT | AGCTTCTTGCAGGGCTTTCA | Start: 1279 |
| Tm | 59.955 | 60.179 | End: 1459 |
| Size | 20 | 20 | 181 |
| Primer 5 details |
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| Sequence | CAAGTTCTCCTCCGGCGTAA | GCGGAGTGGGACCATTGTAA | Start: 1213 |
| Tm | 59.754 | 60.036 | End: 1340 |
| Size | 20 | 20 | 128 |
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