ssr_primer
Primer Details for TRINITY_DN85935_c1_g3_i3 |
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| (T)28: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAGAGGCAGTTCCGTCACTT | TGCCAGAGATGGGACGAGTA | Start: 418 |
| Tm | 59.966 | 60.034 | End: 546 |
| Size | 20 | 20 | 129 |
| Primer 2 details |
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| Sequence | CAGAGGCAGTTCCGTCACTT | TCGTTGGCCTTTCCGAATCA | Start: 418 |
| Tm | 59.966 | 59.964 | End: 566 |
| Size | 20 | 20 | 149 |
| Primer 3 details |
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| Sequence | TACTCGTCCCATCTCTGGCA | TCCACCTCTCTACGTTCGGT | Start: 527 |
| Tm | 60.034 | 59.963 | End: 801 |
| Size | 20 | 20 | 275 |
| Primer 4 details |
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| Sequence | TGATTCGGAAAGGCCAACGA | TCCACCTCTCTACGTTCGGT | Start: 547 |
| Tm | 59.964 | 59.963 | End: 801 |
| Size | 20 | 20 | 255 |
| Primer 5 details |
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| Sequence | TACTCGTCCCATCTCTGGCA | CCTCTCTACGTTCGGTGCTC | Start: 527 |
| Tm | 60.034 | 59.901 | End: 797 |
| Size | 20 | 20 | 271 |
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