ssr_primer
Primer Details for TRINITY_DN85916_c2_g2_i1 |
|---|
| (CT)6: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGATCGTACAAGCCTTGG | GATGGGGTTGGATGAGTGCA | Start: 411 |
| Tm | 59.748 | 60.034 | End: 522 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | CCACTCTGAGAGCTCACAGC | TTGTGAGGGGATGCAAAGCA | Start: 1397 |
| Tm | 60.109 | 60.179 | End: 1498 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTCCCCACTCTGAGAGCTCA | TTGTGAGGGGATGCAAAGCA | Start: 1393 |
| Tm | 59.885 | 60.179 | End: 1498 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AAGGTTCTGCACGATGGTGT | CCAAGGCTTGTACGATCCCA | Start: 231 |
| Tm | 59.891 | 59.748 | End: 430 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | TCTGCACGATGGTGTGTTGT | CCAAGGCTTGTACGATCCCA | Start: 236 |
| Tm | 60.179 | 59.748 | End: 430 |
| Size | 20 | 20 | 195 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGGGATCGTACAAGCCTTGG | GATGGGGTTGGATGAGTGCA | Start: 411 |
| Tm | 59.748 | 60.034 | End: 522 |
| Size | 20 | 20 | 112 |
| Primer 2 details |
|---|
| Sequence | CCACTCTGAGAGCTCACAGC | TTGTGAGGGGATGCAAAGCA | Start: 1397 |
| Tm | 60.109 | 60.179 | End: 1498 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTCCCCACTCTGAGAGCTCA | TTGTGAGGGGATGCAAAGCA | Start: 1393 |
| Tm | 59.885 | 60.179 | End: 1498 |
| Size | 20 | 20 | 106 |
| Primer 4 details |
|---|
| Sequence | AAGGTTCTGCACGATGGTGT | CCAAGGCTTGTACGATCCCA | Start: 231 |
| Tm | 59.891 | 59.748 | End: 430 |
| Size | 20 | 20 | 200 |
| Primer 5 details |
|---|
| Sequence | TCTGCACGATGGTGTGTTGT | CCAAGGCTTGTACGATCCCA | Start: 236 |
| Tm | 60.179 | 59.748 | End: 430 |
| Size | 20 | 20 | 195 |
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