ssr_primer
Primer Details for TRINITY_DN85880_c1_g1_i9 |
|---|
| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTTGTAGGCAGTCACAGC | GGACTCTGTACGATCCGCTG | Start: 3780 |
| Tm | 59.965 | 59.97 | End: 3957 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | CTCTGTACGATCCGCTGCAT | Start: 3780 |
| Tm | 59.965 | 59.969 | End: 3954 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | AACAACTGGGCCAAAGGACA | Start: 3780 |
| Tm | 59.965 | 60.033 | End: 3881 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | GAAAGCTTTGCGCCTGAACA | TCCCATCCAACTGCAAGACC | Start: 2994 |
| Tm | 59.969 | 59.962 | End: 3142 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | TCCGCTGCATTTGGTCAGAT | Start: 3780 |
| Tm | 59.965 | 60.035 | End: 3944 |
| Size | 20 | 20 | 165 |
(A)35: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCTTGTAGGCAGTCACAGC | GGACTCTGTACGATCCGCTG | Start: 3780 |
| Tm | 59.965 | 59.97 | End: 3957 |
| Size | 20 | 20 | 178 |
| Primer 2 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | CTCTGTACGATCCGCTGCAT | Start: 3780 |
| Tm | 59.965 | 59.969 | End: 3954 |
| Size | 20 | 20 | 175 |
| Primer 3 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | AACAACTGGGCCAAAGGACA | Start: 3780 |
| Tm | 59.965 | 60.033 | End: 3881 |
| Size | 20 | 20 | 102 |
| Primer 4 details |
|---|
| Sequence | GAAAGCTTTGCGCCTGAACA | TCCCATCCAACTGCAAGACC | Start: 2994 |
| Tm | 59.969 | 59.962 | End: 3142 |
| Size | 20 | 20 | 149 |
| Primer 5 details |
|---|
| Sequence | ACCTTGTAGGCAGTCACAGC | TCCGCTGCATTTGGTCAGAT | Start: 3780 |
| Tm | 59.965 | 60.035 | End: 3944 |
| Size | 20 | 20 | 165 |
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