ssr_primer
Primer Details for TRINITY_DN85875_c2_g1_i1 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCTCGTCAGGAGGAGCAGAA | CTTTCCGGCTCCGTTTTTCG | Start: 69 |
| Tm | 59.962 | 60.11 | End: 328 |
| Size | 20 | 20 | 260 |
| Primer 2 details |
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| Sequence | TCTCGTCAGGAGGAGCAGAA | GCTCCGTTTTTCGACTCTGC | Start: 69 |
| Tm | 59.962 | 59.836 | End: 321 |
| Size | 20 | 20 | 253 |
| Primer 3 details |
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| Sequence | TTTCGACTGTTGGGCCGTAA | CTTTCCGGCTCCGTTTTTCG | Start: 140 |
| Tm | 59.894 | 60.11 | End: 328 |
| Size | 20 | 20 | 189 |
| Primer 4 details |
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| Sequence | TCGACTGTTGGGCCGTAATC | CTTTCCGGCTCCGTTTTTCG | Start: 142 |
| Tm | 60.109 | 60.11 | End: 328 |
| Size | 20 | 20 | 187 |
| Primer 5 details |
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| Sequence | GCTACGGAGCTTTCGACTGT | CTTTCCGGCTCCGTTTTTCG | Start: 130 |
| Tm | 60.109 | 60.11 | End: 328 |
| Size | 20 | 20 | 199 |
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